Molecular Characteristics of Phytophthora katsurae Using PCR-SSCP Analysis
نویسندگان
چکیده
منابع مشابه
Rapid identification of Phytophthora ramorum using PCR-SSCP analysis of ribosomal DNA ITS-1.
AIMS The primary objectives of this study were to determine if a single-strand conformation polymorphism (SSCP) analysis can be used for rapid identification of Phytophthora ramorum, an important quarantine plant pathogen worldwide, and to further assess the potential of the SSCP technique as a taxonomic tool for the genus Phytophthora. METHODS AND RESULTS SSCP of ribosomal DNA internal trans...
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The method to internally label PCR products with multiple colored fluorescent dyes was developed and applied to multiple fluorescence-based PCR single-stranded conformational polymorphism (MF-PCR-SSCP) analysis. PCR-amplified fluorescent DNA fragments, which were internally labeled by adding fluorescent dUTPs ([F]dUTPs) to the PCR mixture, were heat-denatured and applied to a nondenaturing poly...
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ژورنال
عنوان ژورنال: Research in Plant Disease
سال: 2011
ISSN: 1598-2262
DOI: 10.5423/rpd.2011.17.2.169